Cloned (Comment) | Organism |
---|---|
expression in Shewanella oneidensis | Trichlorobacter lovleyi |
Crystallization (Comment) | Organism |
---|---|
structure at 2.55 A resolution, presence of an arginine residue in the region otherwise occupied by calcium in canonical NrfA enzymes | Trichlorobacter lovleyi |
Protein Variants | Comment | Organism |
---|---|---|
R277A | variant contains all five hemes, less than 3% of wild-type activity | Trichlorobacter lovleyi |
R277K | variant contains all five hemes, less than 3% of wild-type activity | Trichlorobacter lovleyi |
R277Q | variant contains all five hemes, less than 3% of wild-type activity | Trichlorobacter lovleyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA at 1 mM | Trichlorobacter lovleyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
nitrite | pH 7, temperature not specified in the publication | Trichlorobacter lovleyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not required: Ca2+ | Trichlorobacter lovleyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
dynamic light scattering | Trichlorobacter lovleyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichlorobacter lovleyi | B3E641 | - |
- |
Trichlorobacter lovleyi DSM 17278 | B3E641 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, by Strep-tag II affinity chromatography followed by size-exclusion chromatography | Trichlorobacter lovleyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced methyl viologen | - |
Trichlorobacter lovleyi | NH3 + oxidized methyl viologen + H2O | - |
? | |
nitrite + reduced methyl viologen | - |
Trichlorobacter lovleyi DSM 17278 | NH3 + oxidized methyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56196, electrospray ionization, consistent with the mass of the polypeptide chain plus the five heme cofactors but no calcium ion | Trichlorobacter lovleyi |
More | the enzyme crystallizes as a dimer but remains monomeric in solution | Trichlorobacter lovleyi |
Synonyms | Comment | Organism |
---|---|---|
Glov_1042 | - |
Trichlorobacter lovleyi |
NrfA | - |
Trichlorobacter lovleyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | five hemes per monomer | Trichlorobacter lovleyi |